SDS Sample Buffer (6x)

(0.375M Tris pH 6.8, 12% SDS, 60% glycerol, 0.6M DTT, 0.06% bromophenol blue)'''

-combine 3.75ml 1M Tris-Cl, pH 6.8, 6ml glycerol, 1.2g SDS (FW=288.38), 0.93g DTT (FW=154.2), 6mg bromophenol blue. Add water to total volume of 10ml. Store at -20˚C in 0.5ml aliquots.