Pipetting Basics

100 – 1000 microliters

 * Use blue colored pipet tray


 * One’s place does not show on the scale


 * DO NOT go below 200 microliters on the scale

20-200 microliters

 * Use green colored pipet tray

2 – 20 microliters
opposed to black)
 * Use red colored pipet tray
 * Third number on the scale is the ones place after a decimal point (numbers are red colored as

0.5 – 10 microliters

 * Use red colored pipet tray
 * Third number on the scale is the ones place after a decimal point
 * USE between 2 – 10 microliters

0.1 – 2 microliters

 * Use red colored pipet tray
 * 1s, .1s and .01s place is shown

** NOTE: IF TIP OF PIPET TOUCHES CLOTHING, SKIN, COUNTER, ETC. USE A NEW TIP AND TOSS OLD ONE OUT. **

How to use a pipet:
1. Unlock pipet by turning blue knob from the lock label to the unlocked label.

2. Adjust the scale to the amount you wish to pipet by turning the knob on the top.

3. Lock pipet by turning blue knob back to locked label.

4. Push the bottom of the pipet onto a tip until you are sure it is secured

5. Apply thumb on the top knob of the pipet and push down until you full first resistance.

6. Apply the tip into solution you wish to pipet but only enough to be able to obtain solution securely.

7. Release the knob while tip is in solution and then pull back the tip once you have released your thump completely.

8. Hold the tube you wish to pipet your solution into in your other hand in order for you to be able to see what is happening up close.

9. Place the tip in the tube and push down on the knob all the way in order to make sure all the solution has exited the tip

10. Keep your hand pushed down on the knob until you have released the tip from the solution to make sure none of the solution gets sucked back up into the tip

11. Discard the tip in a non-hazardous waste tub.