MOM Lab Plasmid Database

Congratulations! Despite science’s best efforts to derail you, you have successfully created a construct. It has been sequenced and confirmed to be correct. But now you ask, “how do I preserve my work for future generations of MOM lab scientists?”  Well enter it into the database of course!

MOM Lab Plasmid Database


 * 1) Give your Plasmid a MOM Number.
 * 2) Give a brief descriptive name for your plasmid (This can be the name of your .ape file).
 * 3) Enter in the Full  name of the gene


 * 1) Include any truncation or mutation information you have in parentheses
 * 2) Enter in the plasmid backbone.


 * 1) Either the general backbone or the MOM plasmid you used to clone it.
 * 2) <p dir="ltr" style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;">Enter the Gene number where the specific gene can be found


 * 1) <span style="font-size:11pt;font-family:Arial;color:#000000;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;">Common databases: <span style="font-size:11pt;font-family:Arial;color:#1155cc;font-weight:400;font-style:normal;font-variant:normal;text-decoration:underline;vertical-align:baseline;">Genbank<span style="font-size:11pt;font-family:Arial;color:#000000;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;">, <span style="font-size:11pt;font-family:Arial;color:#1155cc;font-weight:400;font-style:normal;font-variant:normal;text-decoration:underline;vertical-align:baseline;">Flybase<span style="font-size:11pt;font-family:Arial;color:#000000;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;">, <span style="font-size:11pt;font-family:Arial;color:#1155cc;font-weight:400;font-style:normal;font-variant:normal;text-decoration:underline;vertical-align:baseline;">Mammalian Gene Collection
 * 2) <p dir="ltr" style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;">Enter other relevant information
 * 3) <p dir="ltr" style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;">Enter notes about the plasmid


 * 1) <p dir="ltr" style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;">I.e. primers used to clone.  CRISPR?  Guide RNA? Expression? Project?
 * 2) <p dir="ltr" style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;">Deposit the .ape file onto the MOM Server and change the name of the file to match the MOM Number.


 * 1) <span style="font-size:11pt;font-family:Arial;color:#000000;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;">In the same folder, deposit all the sequencing files for this construct.
 * 2) <p dir="ltr" style="line-height:1.38;margin-top:0pt;margin-bottom:0pt;"><span style="font-size:11pt;font-family:Arial;color:#000000;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;">Transfer the DNA (preferably the entire miniprep) to a new centrifuge tube labeled with the MOM Number and store in the appropriate -20 degree box.


 * 1) <span style="font-size:11pt;font-family:Arial;color:#000000;font-weight:400;font-style:normal;font-variant:normal;text-decoration:none;vertical-align:baseline;">Please dilute the DNA to 100 ng/ul.  Even at -20 there is minor evaporation.  Over months and years, this can lead to a significant decrease in volume.