Agarose Gel

Then follow the Gel Electrophoresis steps for casting and running your gel.
 * 1) Weigh enough agarose* to make a 1% gel (eg 1g/100mL). *Make sure the agarose used is specifically for making gels, otherwise your gel will start to dissolve when run at 120V.
 * 2) Add 1X TAE buffer to the agar in a flask.**Use a flask large enough that the amount of agar you are making fills it about halfway (for 500mL agarose, use a 1L flask). This will help to ensure the agarose doesn’t overflow when heated.
 * 3) Swirl and heat in microwave to dissolve. (3-4 mins for 500mL). Watch to make sure it doesn’t boil over in the microwave, and periodically take out and CAREFULLY swirl around to ensure all agarose dissolves (you can check by holding up to the light and looking for the disappearance of whitish/translucent chunks). When it is completely dissolved the liquid should be totally transparent.