Sending out DNA for Sequencing

You will have 15uL total sample

You will need to label each tube (PCR sized) KM01, KM02.... etc

You need no more than 800ng of DNA in each sample.

So if your concentration is 100ng/uL --> 800ng/100ng/uL = 8uL of DNA to add

You'll also need to add 2.5uL of your primer (10uM stock)

Then you'll add water for a total of 15uL

So, as an example:

8uL DNA

2.5uL Primerr

4.5uL Water