Mini-Prep

'''***USE NEW ZYMO KIT. FOLLOW INSTRUCTIONS IN PAMPHLET, NOT “SHORTCUT” INSTRUCTIONS. THE BELOW ARE INSTRUCTIONS FOR MINIPREP USING OLD QIAGEN REAGENTS AND COLUMNS.
 * 1) Grow cells in 5 mL of LB with correct selection agent overnight at 37 deg C.
 * 2)  The next morning, spin down 2 mL of each culture at at a time in 2 mL tube for 1 minute at top speed in the large centrifuge. Aspirate out media, taking care not to disturb pellet. RESERVE 500 uL to 1 mL of culture for glycerol stocks.
 * 3) Add 250 uL of Resuspension solution to cell pellet. Pipette VERY GENTLY up and down to resuspend cells. (***It is crucial at this stage to not form bubbles, as this can cause loss of as much as 50% of your plasmid DNA).
 * 4) Add 250 uL of Lysis buffer; invert several times to mix. Incubate at room temperature for 3 minutes.
 * 5) Add 350 uL of Neutralization solution; invert exactly 5 times, no more, no less.(At this stage, a white cloudy substance should be visible; this is your genomic DNA/protein lysate).
 * 6) Spin at max speed in the large centrifuge for 7 minutes. Transfer supernatant to a spin column, taking care not to incorporate any of the cloudy white protein.
 * 7) Spin supernatant through the spin column (Can be done in the small tabletop spinner)
 * 8) Wash TWICE with 750 uL of Wash buffer; spin thru w/ tabletop spinner each time.
 * 9) After removal of second wash, dry column by spinning for 2 minutes at top speed in the large centrifuge.
 * 10) Transfer spin column to a fresh 1.5 mL tube. Add 50 uL of Elution Buffer. Spin for 4 minutes in large centrifuge at top speed to elute plasmid DNA. Check concentration using the nanodrop.