Cloning Steps

10 Steps of Successful Cloning:
 * 1) PCR vector and insert(s), verify with Gel, DPN1 digest, and then purify DNA
 * 2) Gibson
 * 3) Transform competent cells (two protocols listed: 1 and 2)
 * 4) Plate cells on selective media
 * 5) Plates have colonies
 * 6) Colony PCR on 28 colonies, run gel
 * 7) Pick 2 colonies, grow in LB
 * 8) Miniprep
 * 9) Sequence DNA
 * 10) Smile!