Gel Purification

Cut DNA out of gel using the UV-cutting tray in the printer room, make sure to use correct safety equipment.

Place in a 2 ml tube (you may need to use two or more tubes, so plan accordingly)

Add solubilization buffer to cover gel (protocol recommends that you use a specific volume of buffer per volume of gel)

Place on 55C heat block

Check and invert tube every 3-5 minutes until melted

Add all liquid to a column filter tube and centrifuge at max (discard liquid)

Wash 2X with 200ul wash buffer (centrifuge 30s at max and discard liquid)

Dry spin centrifuge 1 min at max speed

Transfer column to new 1.5ml tube

Add 50ul elution buffer (that was kept warm on 50C heat block)

Incubate at room X 10min

Centrifuge max speed X 3min

Check concentration and label tube with DNA construct (like APE file), date, and [concentration]