Growing Bacterial Colonies in LB

In order to grow bacterial colonies (aka "overnight" clones):
 * 1) Obtain 15mL tubes meant for shaking overnight, if you're not sure which ones they are you can ask someone. Label each tube with the construct (ape file info), clone number, date and cell type (XL10, BL21 etc.).
 * 2) Get the correct antibiotic (for your construct) from the -30C, defrost in the 37-degree water bath and keep on ice.
 * 3) Ethanol your benchtop and once it has dried, start a flame with the bunsen burner to keep your area sterile. *NOTE: NEVER leave your bench while your flame is on!!! If you need to grab something, close all of your caps and/or plates and turn off your flame.
 * 4) Using a pipette man, add 5mL of LB media to each tube.
 * 5) Next, add 1x your antibiotic, so 1000x Kanamycin would be 5uL in 5mL of LB. Make sure to add it directly into the LB media (don't release the small volume while your tip is in the air).
 * 6) Then, using a 20uL pipette (set the volume to 10uL or less so bacteria does not get on your pipette) with a new pipette tip for each colony, pick each colony and place it in its respective tube.
 * 7) Once you have all of the tubes ready, place in the 37-degree shaker overnight (only for 16-20 hours).