Transformation (Gibson)

Transformation Protocol: 
 * 1) Add half* of your Gibson reaction to 50uL of cells. *don’t exceed 5uL of Gibson reaction in 50ul of

cells


 * 1) Incubate on Ice for 20 min.
 * 2) While incubating, fill the 42 heat-block with water
 * 3) After the 20 minute incubation on ice, heat shock at 42degC for 50 sec
 * 4) Place the tube directly back on ice
 * 5) Add 500uL SOC medium and incu-shake at 37degC for 2 hrs.
 * 6) Plate 50uL and incubate plates at 37degC incubator overnight.
 * 7) In the morning, remove your plates and store in the 4C.