K560 Media for BL21 Protein Expression

In 1.5L of MiliQ water:

30g Tryptone

22.5g Yeast Extract

12g NaCl

3g Na2HPO4 (Sodium Phosphate)

1.5g KH2PO4 (Potassium Phosphate)

Autoclave on liquid cycle.

Once cool to the touch, you can add antibiotic. Then add:

12.5mL of 20% glucose (filtered)

You can make 20% glucose by dissolving 8g of glucose in 40mL of water and then filtering it.