PCR Purification

Add 5X binding buffer to DNA in 1.5ml tube

I.e. if you have 95ul of DNA add 95X5= 475ul binding buffer

Pipette up/down until mixed completely

Transfer to spin column

Centrifuge at max speed X 30s (discard liquid)

Wash 2X with 200ul wash buffer (centrifuge 30s at max and discard liquid)

Dry spin 1min at max speed

Transfer column to new 1.5ml tube

Add 50ul elution buffer (that was kept warm on 50C heat block)

Incubate at room X 10min

Centrifuge max speed X 3min

Check concentration and label tube with DNA construct (like APE file), date, [ concentration]