DPN1 Digest

For 100uL Reaction (Two 50uL PCR reactions):

Combine your duplicate PCR reactions into one PCR tube (with 5uL of DNA run on a gel, there should be 45uL left, so 90uL total).

You'll need FD Buffer and DPN1 from the -30, defrost the FD buffer in the 37-degree water bath and keep both on ice.

Add enough 10X fast-digest (FD) buffer to make 1X, so 10uL.

Add 1ul DPN1

Pipette up and down to mix

Incubate in 37C water bath for 15min-1hr

Then purify using the PCR purification kit above the microwave.