Glycerol Stock

Transform DNA to BL21 cells (-80 Box labeled 60, colorful tubes)

Place 50ul of cells in 1.5ml tube

Place 0.5ul of Correct clone sequenced DNA in tube

Place on ice X 15min

Place in 42C bath X 50s (Make sure there is diH20 in well used)

2min on ice

Add 500ul SOC (under flame)

Place on shaker 37C 270-290RPM for 75min

Plate to pre-warmed (at 37C) plate with correct antibiotic

10ul on one plate and 25ul on other

Grow overnight at 37C

Pick one colony and grow overnight on 37C shaker (5ml LB + 5ul antibiotic “1X” + colony DNA)

Place 900ul of solution grown overnight + 900ul 50% glycerol stock in 1.8ml cryotube

Label tube

Place in -80 (in correct box)