Protocols

ATPase Assay

ATPase Assay (written by: Danielle)

Bacterial Cell Transformation

Baculovirus Production with Bac-to-Bac (Invitrogen)

Cloning Steps

Colony PCR

DDB Pull Down

DPN1 Digest

Emulsiflex

Gel Electrophoresis

Gel Purification

Gibson Reaction

Growing Bacterial Colonies in LB

KOD PCR

Making Competent Cells (using Zymo Mix and Go)

Mini-Prep

PCR Purification

Pipetting Basics

Processing Clones from Whatman FTA Discs (From DGRC)

Protein Expression in Bacterial BL21 Cells

Protein Purification (Syringe/Pump)

Sequencing

Streptactin Column Regeneration

TIRF Microscopy

Transformation (Gibson)

Q5 PCR

Making de-tyrosinated Microtubule for TIRF by VASHI plus SVBP